Acquisitions Management Operations Branch, NIAID, 6003 Executive Blvd, Solar Building, Room 1C38, Contracts Section, Bethesda, MD 20892-7605

R -- MANUFACTURE OF SYNTHETIC PEPTIDES SOL NIAID-DIR-98-56 DUE 083198 POC Ivan Hernandez, 301-496-3878, Karin E.Eddy, Contracting Officer, 301-496-3878 The Contractor will provide the Government with the following: 1. Provide the NIH-NIAID with synthetic peptides and peptide-carrier protein conjugates in accordance with requested quantity and quality, and within specific time periods. Contractor will provide the Government with 250 to 300 synthetic peptides per year in 4 ranges of purity, as determined by an analytical HPLC analysis: Unpurified; over 75% purity; Over 85% purity; OVER 95% purity. Peptides shall be delivered according to specified amounts that will fall into 6 ranges: 5-15mg; 16-30mg; 31-50mg: 51-100mg; 101-150mg; 151-200mg. Some of the ordered synthetic peptides will require a chemical modification of the peptide molecules, that would typically involve the following: Amidation at C-Terminal; Acetylation at N-Terminal; Biotinylation of the N-Terminal; Incorporation of the phosphorylated Amino Acids, such as Phospho-Tyrosine, phospho-Serine and phospho-Threonine. Based on NIAID experience it is anticipated that a number of requests for structurally modified peptides would be in a 10-15% of all requests, with majority of these requests invoving amidation at C-terminal and acetylation at N-Terminal. 2. Contractor will also provide the Government with peptide/carrier protein conjugates, obtained by coupling the synthetic peptide to the carrier proteins such as Keyhole Lympet Hemocyanin (KLH), Bovine Serum Albumin (BSA), or other proteins typically used for production of bioconjugates. Typically, a part of the ordered synthetic peptide will have to be coupled to the carrier protein, with the excess of peptide shipped in the free form. In each case the synthetic peptide, carrier protein, amounts needed, and a method of conjugation and/or the conjugating reagent will be specified in the order. All prices quoted for goods delivered to Government sites must include shipping charges. The Contractor shall develop a pricing schedule that will encompass all proposed ranges of synthetic peptide purities, requested amounts, and sequence lengths. It is anticipated that one award will be made for one base year plus four (4) additional one-year options. RFP 98-56, will be available on or about July 29, 1998, with an estimated closing date of August 31, 1998. This requirement is classified as a 100% competitive set-aside for small business concerns (see Note 1). Copies of RFP 98-56 may be obtained by written requests to Ivan Hernandez at the address listed above. Posted 07/08/98. (0189)

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