SOURCES SOUGHT
65 -- iBright FL 1000 imaging System NOTICE of INTENT to SOLE SOURCE
- Notice Date
- 3/6/2019
- Notice Type
- Synopsis
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Veterans Affairs;Network Contracting Office 2;James J. Peters VA Medical Center;130 West Kingsbridge Road;Bronx NY 10468-3904
- ZIP Code
- 10468
- Solicitation Number
- 36C24219Q0423
- Response Due
- 3/13/2019
- Archive Date
- 3/23/2019
- Point of Contact
- (914) 737-4400 ext 2052
- Small Business Set-Aside
- N/A
- Description
- Statement of Work-iBright FL1000 Background: Essential hypertension (eHTN) affects millions of adults in the U.S. and contributes to end organ damage including kidney failure. The epidemic of eHTN is, in part, related to environmental changes, and, the high sodium (Na) and cholesterol (chol) diets consumed in the U.S. The link between Na consumption and eHTN is established; however, the relationship between chol and eHTN is not well understood. Chronic, long-term hypercholesterolemia leads to arteriolosclerosis and diminished vascular compliance predisposing to eHTN; however, recent evidence suggests that acute, short-term chol ingestion augments renal Na avidity to raise blood pressure (BP). Hypercholesterolemia promotes incorporation of chol into lipid rafts (LRs) of the plasma membrane (PM) which raises PM viscosity and induces diseases such as diabetes (by suppressing insulin secretion) and diabetic nephropathy. The contribution of chol to organ dysfunction is underscored by the observation that chol chelation by cyclodextrin improved insulin secretion and diabetic nephropathy while treatment with statins had no effect. We hypothesize that chol incorporation into the lipid rafts and plasma membrane of the kidney collecting leads to enhanced epithelial sodium channel dependent trans epithelial sodium transport which contributes to the development of hypertension. Objective: We seek to purchase an IBright Imaging system, equipment that permits the imaging of proteins on western blots, nucleic acids in gels, and fluorescence imaging of multiple proteins on a single western blot. Place of Performance: Northport VAMC, Building 62. Scope of Tasks/Product Specifications: Our goal is to measure chemiluminescence on western blots to determine relative protein concentrations in collecting duct cell and kidney tissue. These studies will help to determine whether there are changes in cation channel concentration and natriuretic proteins in kidney when mice have been fed a high cholesterol diet. The iBright system is the best system we tested for our needs. See below for the details. 1. iBright is unique that it comes with a license free Image analysis application that is available both as a local desktop supported version and a cloud hosted version. The cloud based analysis application allows the customer the ability to access, analyze, quantitate, and share their images from any computer with internet access. There is no limit to the number of users per instrument and each user received 10gb of storage space for their utilization. 2. The iBright FL1000 and CL1000 imaging systems are equipped with a high resolution 9.1-megapixel, high sensitivity, scientific CCD camera. The high resolution of the camera combined with a wide range of binning options (1x1 to 8x8) allows the user to readily adjust the system, based on the needs of their application, to provide high resolution images and exceptional speed and sensitivity. 3. Smart Exposure: Smart Exposure is a patent-pending interactive tool used to evaluate and optimize imaging exposure time before acquiring a Western blot image. The Smart Exposure button automatically calculates the exposure time of the Western blot with maximum dynamic range and minimal pixel saturation from a short exposure image and displays what a projected image of this optimal exposure would look like. The feature displays real-time changes of the projected Western blot image with adjusted exposure time and allows further exposure-time refinement through user input. 4. Mechanical sample alignment: The iBright imaging systems utilizes a mechanical tray to physically rotate a sample aligning it with the camera before image acquisition. This patent pending technology eliminates the need for digital rotation which modifies the image resulting in distorted intensity values during image analysis. 5. Multi-frame identification and Auto Analysis: iBright imagers have proprietary built-in algorithms that can uniquely identify multiple analysis frames and then assigns and quantifies lanes and bands for each analysis frame independently. This allow multiple gels or membranes to be imaged and analyzed simultaneously with minimal user input and manipulation. 6. Molecular Weight Overlay feature: The proprietary molecular weight marker (MWM) overlay feature allows users to perform molecular weight determination using a colorimetric molecular weight marker in the membrane channel and combining it with the corresponding chemiluminescent or fluorescent image. To enable this, the imaging system automatically acquires both a visible and a detection image and then digitally combines them to enable visualizing both the molecular weight markers and the western blot on the same image. 7. Low Profile Imaging and Low-Profile Zoom. The iBright imaging systems utilize an internal light path design that allows maximum sample viewing area in a smaller instrument size (patents pending). This allows iBright to minimize the utilization valuable bench space in the laboratory while still being able to image multiple gels and blots simultaneously. Government-Furnished Property, Material, Equipment, or Information (GFP, GFM, GFE, or GFI): Not Applicable Security Software to be used to analyze data and images taken from animal samples as part of VA approved research protocols. No PII/PHI The C&A requirements do not apply, and a Security Accreditation Package is not required NOTE: THIS NOTICE WAS NOT POSTED TO FEDBIZOPPS ON THE DATE INDICATED IN THE NOTICE ITSELF (06-MAR-2019); HOWEVER, IT DID APPEAR IN THE FEDBIZOPPS FTP FEED ON THIS DATE. PLEASE CONTACT 877-472-3779 or fbo.support@gsa.gov REGARDING THIS ISSUE.
- Web Link
-
Link To Document
(https://www.fbo.gov/spg/VA/BroVANAP/VAMD/36C24219Q0423/listing.html)
- Place of Performance
- Zip Code: 11768
- Record
- SN05241418-F 20190308/190306230020 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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