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FBO DAILY - FEDBIZOPPS ISSUE OF DECEMBER 12, 2013 FBO #4401
SOLICITATION NOTICE

66 -- Brand Name LSM 780 Confocal Microscope with 34 Channel Spectral Detection for the National Cancer Institute (NCI), Center for Cancer Research (CCR)

Notice Date

12/10/2013
 

Notice Type

Presolicitation
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bldg 1050, Frederick, Maryland, 21702, United States
 

ZIP Code

21702
 

Solicitation Number

N02RC44103-57
 

Archive Date

1/8/2014
 

Point of Contact

Reyes Rodriguez, Phone: 240-276-5442, Terry L. Galloway, Phone: 240-276-5384
 

E-Mail Address

reyes.rodriguez@nih.gov, gallowaytl@mail.nih.gov
(reyes.rodriguez@nih.gov, gallowaytl@mail.nih.gov)
 

Small Business Set-Aside

N/A
 

Description

National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Receptor Biology and Gene Expression (LRBGE), plans to procure on a sole source basis the LSM 780 Confocal Microscope with 34 Channel Spectral Detection from Carl Zeiss Microscopy, LLC, One Zeiss Drive, Thornwood, NY 10594. This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and will be made pursuant to the authority in FAR 13.106-1(b)(2) and 13.501-(a)(1) using simplified acquisition procedures for commercial acquisitions. The North American Industry Classification System code is 334516 and the business size standard is 500 employees. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. It has been determined there are no opportunities to acquire green products or services for this procurement. Delivery Date: 16 weeks from date of award. The CCR Fluorescent Microscopy Core Facility was established by the NCI Laboratory of Receptor Biology and Gene Expression (LRBGE) in 1998. The core facility provides state-of-the-art confocal microscopy, deconvolution microscopy and digital imaging to CCR investigators to perform high quality basic, translational and clinical research in cancer biology and to train students, residents, and fellows in state of the art methods in optical microscopy. The core facility also provides services and opportunities for collaborative research to investigators throughout the NCI as well as other NIH Institutes. Key application areas in the facility include procedures for single, double and triple label imaging of fixed and living specimens. Long-term Time-lapse imaging of live specimens is also a common application. In addition to these procedures, the facility personnel and users have special expertise in several advanced procedures. These include methods for the determination of molecular interactions within live cells. LRBGE is using two different approaches to measure these interactions: fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET). Finally, a number of facility users are studying the cellular response to DNA damage, and these researchers wish to use confocal microscopy to induce DNA damage at defined sites within the cell nucleus, and then study the cellular response to that damage. Contractor Requirements are: Microscope: * Inverted Microscope stand with Fully motorized 6 position compatible objective nosepiece and 6 position push and click motorized fluorescence filter turret * Motorized integrated Z focus with a step size of 10nm and travel range of 25mm * Motorized X,Y stage with high accuracy and reproducibility. Stage should include joystick but be fully software integrated. * TFT touch panel independent of microscope stand with focus control and access to all microscope motorized functions. Can be placed conveniently by computer and monitors. * Objectives must include equivalent or better EC Plan Neofluar 5x/0.16, Plan Apochromat 40x/1.4, Alpha PlanApo 63x/1.46,, Alpha PlanApo 100x/1.46 Manually controlled Metal Halide Fluorescence burner with variable intensity output. * High Resolution DC Closed Loop Scanning Stage with Joystick and Integrated Computer Control including Universal Specimen Holder Suitable for variety of slide and round dishes upto 60mm,..5u reproducibility over full travel range. * Definite Focus - Integrated focus which maintains focus during long term live cell imaging. * System must include full Environmental incubation system that includes heating and CO2 to maintain optimal live cell conditions for multi day experiments. Features of High Sensitivity Laser Scanning Confocal Microscope: * Must Incorporate a 34-Channel Quasar(Spectral) GaAsP Detector, consisting of calibrated 32-Array GaAsp and two flanking single PMT detectors, enabling image acquisition with freely definable emission spectra and advanced spectral acquisition and analysis Spectral GaAsp detector allows increased QE(>45%) over standard PMT detectors, critical for our low light fluorescence applications. * System must include the following laser lines 355, 405, 458,488,514,561,594,633 * Hardware must support simultaneous acquisition from 34 channels plus TPMT and 2 additional detectors for up to 37 simultaneous 16 bit inputs. * Spectral GaAsP should allow image collecting in photon counting mode for single molecule applications or integration mode for standard fluorescence imaging. * Fluorescence Correlation Spectroscopy (FCS) to analyze single molecules with GaAsP detector. Up to 6 channels can be used in FCS mode, providing greater flexibility in stainings and samples. * Able to perform FCS analysis with an actively cooled, photon counting GaAsP detector. * Must be compatible with existing c-Apochromat 40x 1.1 NA FCS lens. * Incorporates Primary Dichroioc Beam Splitter(Twin Gate) at 10 degree position, you achieve Extremely HIGH laser suppression on the order of 6-7 OD allowing superb reflection free imaging. The System omits all secondary dichroics for high efficiency emission collection. * Point Scanner must obtain at least 8 fps at 512x512 and e.g. 250 fps at 512x16 * Digital gain (noise free contrast enhancement prior to bit depth conversion).in addition to standard master gain pedestal. * Free selection of emission bands for detection, omitting secondary dichroics or emission filters. Must be possible to set the emission bands over laser lines and still have efficient laser blocking to obtain superior signal to noise with low light fluorescent samples. * Freely rotatable scan field (0-360 degree in 0.1° steps) with user definable size and shape due to independent galvo-mirrors. * Real time electronics control offers more flexibility in managing image data in parallel WHILE ACQUIRING! Users can image process and review, not only previously collected images, but also the image data set currently being acquired without interfering with the image acquisition. Parallel image Acquisition and Analysis on the Confocal Platform is a requirement. * Multiple (up to 99) freely definable real regions of interest (rROIs). Pixel precise control of the laser intensity and signal detection. Prevention of photo damage outside the rROIs boundaries necessary for work on living cells, FRAP, uncaging, photoactivation and photoconversion experiments. * Individual AOTF settings (line selection and attenuation) per rROI. * Use up to 8 laser lines simultaneously with full power possible from each laser line. * System must offer at least 15 different scan speed levels (28 levels including bi-directional scans) for the wide variety of specimens and applications we will design. This is especially critical for our single molecule experiments. * It must be possible to select different scan speeds for bleach ROIs and image frame. * Variable scan field size (hardware-zoom) from 0.6 to 40 in 0.1 steps for magnifications until the optical limits of resolution, with a scan field of 20 mm diagonally in the intermediate image plane. * Image format from 4x1 to 6144x6144 pixels in up to 35 detection channels simultaneously with up to 16 bit depth (65536 gray values) in all channels. (8, 12 OR 16 bit user selectable bit depth.) * Spline scan for excitation and data acquisition along a curved line, essential for measurements along biological samples (e.g. cell membranes, cell processes). * Multitracking for the prevention of cross-talk by linewise or framewise switching of the laser excitation line, essential for multi-fluorescence applications and co-localization studies. * Automatic brightness compensation during the acquisition of z stacks by stepped incrementation of the excitation (laser intensity) and/or the detection gain (detector sensitivity). Must support at least a 5 point incrementation. * Advanced time-series module with automatic bleach and direct trigger- control to design the entire experimental protocol. All scan strategies can be applied (line, spline, frame, z-line, z-spline, z-stack, rROIs, z-rROIs) in the time series. * Absolute linear scanner movement to ensure equal pixel dwell-times as a prerequisite for any quantitative studies. * Calibration and linearization of the 32-Array GaAsp detector and the two flanking single PMTs, all 34-channels can be used for spectral acquisition and analysis. * 34-Channel GaAsP detector (GaAsp-array with 32 PMTs and two flanking single PMTs) in combination with the highly efficient holographic grating to provide an even spectral dispersion over the entire visual spectral range. * Spectral recycling loop efficiently recovers non-separated light, redirecting it onto the holographic grating. * Fast parallel acquisition in one single scan of the entire spectral range as a lambda stack (391-749 nm) covered by the 34-Channel QUASAR Detector. (Suitable for spectral analysis in living cells, e.g. colocalization studies and FRET-experiments). Up to 10 different fluorescent dyes can be imaged and separated simultaneously. * Spectral bandwidth for lambda stack acquisition must be selectable by the user. A full spectral image is used for unmixing (suitable for spectral imaging of living cells and later unmixing without any spectral distortions). * Reproducible high resolution spectral data acquisition with 3.2 nm or 4.9 nm steps through sequential spectral scanning in addition to the 10nm parallel spectral scanning. * Online Fingerprinting: Image channel definition not by emission bands but by reference spectra and live processing unmixing during image acquisition. * Spectra can be created from a Lambda stack. * Free combination of manually defined, automatically extracted and spectra stored in the database is possible to perform unmixing on a lambda stack. * Potential to use the entire emission spectrum for dyes for detecting with much higher signal to noise ratios (in contrast to detection with bandpass techniques). * Optics are designed for high transmission and aberration free imaging for 350 - 1100 nm. * Pigtailed visible Lasers, are easily upgradeable. Plug and play bayonette mounting for easy maintenance and reliable, reproducible operation. No mirror alignment required for lasers. * Quantitative Colocalization is a part of the basic software with an interactive link between image display, scattergram and data table. The software includes interactive or automatic determination of thresholds, quantitative colocalization analysis with parameters like number of pixels, mean intensities +/-SD, colocalization coefficients, weighted colocalization coefficients, overlap coefficient after Manders, correlation coefficients, export of the analysis result and extraction of the colocalizing structures e.g. from image stacks. * Transmitted detector must be included. Combination of a transmitted- light illuminator (e.g. a halogen lamp) with one of the detectors, fluorescent images can be superimposed with non-confocal transmitted light images. Software Options: * FRAP acquisition and analysis must be software integrated * FCS -integrated Fluorescence Correlation software for single molecular analysis must be included * Multiple Time Series software package permits complex, combined time series with changing application configurations, autofocus and bleach functions. * Tiling and Multiposition Scanning fully integrated into software. * Smart Setup Software for easy hardware control to adapt system configuration according to chosen dyes from a large database of fluorochromes. Different acquisition modes are suggested (e.g. Fastest and Best Signal). * Optimal hardware settings for Channel unmixing are suggested within the Smart Setup Software tool. * System should include self-test tool, with an easy to use maintenance user interface. In combination with a calibration objective Allows automated pinhole adjustment and automatic scanner calibration, securing optimal system performance. * Innovative concept for one touch button for reproducibility of experiments for multi-user environments via a REUSE function allows reactivation of all acquisition parameters necessary to reproduce an experiment (each image stores the entire method). * User-specific Workspace setups can be stored within the ZEN software according to the users needs and application. * Zen Desk full Second copy of software must be included with all capabilities for offline analysis, Software must be fully functional for offline processing as main system. * ZEN Light must also be available to all users for simple process, measure or display your LSM images on an offline workstation. Order and manage your image data using the ZEN File Browser. Use all acquisition parameters and share images through a variety of Export formats. Simple 3D and interactive measurement supported. This notice is not a request for competitive quotation. However, if any interested party, especially small business believes it can meet the above requirement, it may submit a proposal or quote for the Government to consider. The response and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Responses must be received in the contracting office by 11 AM EST, on December 24, 2013. All responses and questions must be in writing and faxed to 240-276-5399 or emailed to Reyes Rodriguez, Contracting Specialist via electronic mail at reyes.rodriguez@nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA) through sam.gov. Reference: N02RC44103-57 on all correspondence.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/FCRF/N02RC44103-57/listing.html)
 

Place of Performance

Address: 9000 Rockville Pike, Bldg 41, Bethesda, Maryland, 20892, United States

Zip Code: 20892
 

Record

SN03249072-W 20131212/131210234406-70f1a84a65d3b6e996dca85fe01619ed (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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