SOLICITATION NOTICE
B -- PathHunter Cell Plating Reagent
- Notice Date
- 4/2/2012
- Notice Type
- Presolicitation
- NAICS
- 541712
— Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(TR)-2012-107-RSF
- Archive Date
- 4/27/2012
- Point of Contact
- Rashida S. Ferebee, Phone: 3014352605
- E-Mail Address
-
ferebeers@nhlbi.nih.gov
(ferebeers@nhlbi.nih.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI), Office of Acquisition (OA) on behalf of the National Center for Advancing Translational Sciences (NCATS) intends to negotiate and award a purchase order on a noncompetitive sole source basis to DiscoveRx Corporation, 42501 Albrae Street, Freemont CA 94538 for the purchase of Eighteen (18), PathHunter® Cell Plating Reagent (500mL) and two (2) PathHunter® Detector Kits (2400mL). BACKGROUND The mission of the NCATS is to apply the tools of small molecule screening and discovery to the development of chemical probe research tools, for use in the study of protein and cell functions, and biological processes relevant to physiology and disease. NCATS will optimize biochemical, cellular and model organism-based assays submitted by the biomedical research community; perform automated high-throughput screening (HTS); and perform chemistry optimization on confirmed hits to produce chemical probes for dissemination to the research community. PRODUCT DESCRIPTION 1. Quanity: Eighteen (18), PathHunter® Cell Plating Reagent,500mL 2.Quantity: Two (2), PathHunter® Detector Kits, 2400mL SOLE SOURCE DETERMINATION The Principal Investigator and NCATS tested different assay formats to measure selective signaling of the D2 dopamine receptor through β-arrestin. The only assay format that met the standard assay robustness criteria for HTS was the DiscoveRx assay. The DiscoveRx D2 dopamine assay for β-arrestin signal uses the DiscoveRx PathHunter® technology. In this assay system, β-Arrestin is fused to an N-terminal deletion mutant of β-gal (termed the enzyme acceptor of EA) the GPCR of interest is fused to a smaller (42 amino acids); weakly complementing fragment termed ProLink TM. In cells that stably express these fusion proteins, ligand stimulation results in the interaction of β-Arrestin and the ProLink-tagged GPCR, forcing the complementation of the two β-gal fragments and resulting in the formation of a functional enzyme that converts substrate to detectable signal. NCATS has done extensive work to miniaturize the assay to 1536-well format and demonstrated that it produces robust data using the HTS capabilities at NCATS by doing pilot screens with small compound collections. The current D2 DiscoveRx assay is ready for HTS at NCATS. At this moment, there is no alternative assay available to measure D2 dopamine β-arrestin signaling that would allow NCATS to screen of a large collection of compounds (>350,000). The D2 dopamine receptor β-arrestin assay from DiscoveRx consists of a proprietary engineered cell line (PathHunter® D2 CHO K-1) and companion proprietary assay detection kit (PathHunter® Detection Kit). PathHunter TM ß-arrestin GPCR assays detect primary rather than secondary activation events, offering less opportunity for off-target effects. The assays typically also provide larger assay windows than calcium or cAMP and can be run in the same well as the latter. DiscoveRx's Flash Detection Kits provide a simple, homogeneous gain-of-signal assay for measurement of enzyme fragment complementation (EFC) activity in live cells. PathHunter Detection Reagents provided by DiscoveRx are designed for use with their PathHunter Cell Lines expressing both the complementing fragments of β-galactosidase (β-gal). Additional attributes of the DiscoveRx items are that the PathHunter Detection Kit was designed to measure intracellular EFC activity within 2 hours after addition of detection reagents. This kit is ideal for use with any standard benchtop luminometer. The PathHunter Flash Detection Kit was designed to measure interacellular EFC activity in less than one minute after addition of detection reagents. As a result, receptor activation can be detected very rapidly with minimal disruption of cell biology. This kit is ideal for instruments with onboard fluid handling and rapid chemiluminescent data acquisition, such as Hamamatsu FDSS6000 that is currently in use by our labs. These cell lines are engineered to express the complementing fragments β-gal in different cellular compartments. PathHunter® β-Arrestin assays detect GPCR activation by measuring the interaction of β-Arrestin with activated GPCRs. A small, 42 amino acid enzyme fragment, called ProLink was appended to the C-terminus of the GPCR. Arrestin was fused to the larger enzyme fragment, termed EA (Enzyme Acceptor). Activation of the GPCR stimulates binding of arrestin and forces the complementation of the two enzyme fragments, resulting in formation of a functional β-gal enzyme capable of hydrolyzing substrate and generating a chemiluminescent signal. In the PathHunter Nuclear Translocation Assays, EA is localized in the cell nucleus and the small complementing PK fragment is expressed as a fusion protein to a target of interest in the cytoplasm. When a signaling pathway is activated a cascade of events ensues, resulting in the translocation of the target protein to the nucleus and forced complementation of the two β-gal fragments and formation of a functional enzyme that hydrolyzes substrate and generation of a chemiluminescent signal. The PathHunter® Cell-Based Kinase Assays monitor the interaction of wild type, full length receptor tyrosine kinase proteins with SH2 phosphotyrosine binding domains using EFC. This assay does not require antibodies or washes and can be easily adapted to a one-step HTS-friendly assay. These PathHunter® Mitotic Index Assays monitor mitotic activity in live cells by measuring the complementation of nuclear and cytoplasmically localized β-gal fragments. During mitosis, the nuclear envelope degrades and allows the two β-gal fragments to complement in the cytoplasm, forming active enzyme and generation of chemiluminescent signal. Any changes toNCATS' original protocol will cause costly delays time and effort. This project was started using these products from DiscoveRx Corp and these requested reagents are needed in order to finish this project. NCATS cannot afford to spend any time looking for potential alternative suppliers as this will be too expensive to the government and would be detrimental to the data already created under these requested variables. These are trademarked items and not available from any other sources. Industry Classification (NAICS) Code is 541712, with business size standard of 500 Employees. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001) and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-54 (November 2, 2011). This acquisition is set-aside for small business. This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this synopsis, by April 12, 7:30 a.m. Eastern Standard Time. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Inquires to this announcement, referencing synopsis number, NHLBI-CSB-(TR)-2012-107-RSF may be submitted to the National Heart, Lung and Blood Institute, Office of Acquisition, Procurement Branch, 6701 Rockledge Drive, Suite 6145, Bethesda, Maryland 20892-7902, Attention: Rashida Ferebee. Response may be submitted electronically to Rashida.ferebee@nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(TR)-2012-107-RSF/listing.html)
- Place of Performance
- Address: NIH/NCATS, 9800 Medical Center Drive, Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN02711841-W 20120404/120402235415-5673efb16e21615d1a6ac24c04817304 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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