SOLICITATION NOTICE
66 -- Gradient Station
- Notice Date
- 2/9/2012
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HL)-2012-069-JML
- Archive Date
- 2/28/2012
- Point of Contact
- Jonathan M. Lear,
- E-Mail Address
-
learj@nhlbi.nih.gov
(learj@nhlbi.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisitions (OA), intends to negotiate and award a purchase order on a noncompetitive sole source basis to BioComp Instruments, Inc., 650 Churchill Row, Fredericton, NB, CAN E3B 1P6 for the following equipment: -BioComp Gradient Station The Laboratory of Ribonucleoprotein Biochemistry in NHLBI conducts research on the function of messenger RNA-protein complexes in health and disease. The gradient station is necessary for the formation and fractionation of density gradients. Density gradient centrifugation is an important technique for the biochemical analysis of mRNAs. These experiments require reproducible formation of gradients of sucrose, glycerol, or other solutions, followed by ultracentrifugation and collection and analysis of fractions. In particular, the experiments require gradient fractionation followed by mass spectrometry to identify proteins present in individual fractions. Because mass spectrometry is a highly sensitive technique, it is essential that samples generated both within a single experiment and across several experiments be as similar as possible. The gradient station is a unique instrument that combines and automates making reproducible gradients and collecting very small, high resolution fractions which are essential for NHLBI studies. The following specifications are necessary with this particular equipment: -Combined instrument for making reproducible gradients and fractionating them after ultracentrifugation; -Automated, programmable gradient making capability; -Compatibility with a large number of solutes; -Simultaneous formation of six (6) gradients for optimal reproducibility; -Automated high resolution gradient collection (fractionating) capability; -Built-in monitoring of fraction volume and position; -In-line UV monitoring and fraction collection with digital data collection; and, -Small footprint to maximize limited bench space. The sole source determination is based on the fact that this gradient station is the only commercial system available to generate and analyze density gradients in a single package. The gradient forming function is capable of producing six (6) identical gradients in as little as forty (40) seconds, with reproducibility that is not possible in manual gradient formation. This reproducibility is essential for the accurate comparison of multiple samples for analysis using biochemical methods and mass spectrometry. The fractionator module uses a patented system to draw gradient functions as small as ten (10) microns thick, resolutions that are impossible using manual protocols. The fractionator monitors the position of the meniscus, a unique feature important for sample-to-sample reproducibility. In-line UV monitoring and fraction collection speeds data collection and allows detailed analysis of polyribosome profiles, in a digital format. Together, these attributes are essential for the research program of the laboratory, as the work requires precise fractionation of sub-populations of RNA-protein complexes in a highly reproducible manner. The gradient station is the only available instrument that is capable of fulfilling this need. The delivery point is the National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI), Bethesda, MD. The North American Industry Classification System (NAICS) Code is 334516, Analytical Laboratory Instrument Manufacturing, and is not a small business set aside. This acquisition is being conducted under FAR Part 13, Simplified Acquisition Procedures, therefore the requirements of FAR Part 6, Competitive Requirements, are not applicable and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 2005-55 (February 2, 2012). Interested parties may identify their interest and capabilities in response to this synopsis by February 13, 2012 at 9:00am Eastern Standard Time. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurements. Inquiries to this announcement, referencing synopsis number NHLBI-CSB-(HL)-2012-069-JML, may be submitted to the National Heart, Lung, and Blood Institute, Office of Acquisitions, COAC Services Branch, 6701 Rockledge Drive, Room 6151, Bethesda, Maryland 20892-7902, Attention: Jonathan Lear. Responses may be submitted electronically to learj@nhlbi.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HL)-2012-069-JML/listing.html)
- Place of Performance
- Address: National Institutes of Health/NHLBI, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN02672697-W 20120211/120210114821-f1ff5ef548535ad3068b3fe967226695 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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