SOLICITATION NOTICE
66 -- SPRIworks Fragment Library Kit I-50
- Notice Date
- 10/29/2010
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-PB-(HG)-2011-005-DLM
- Archive Date
- 11/17/2010
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) intends to negotiate and award a purchase order on a noncompetitive sole source basis to Beckman Coulter, 200 South Kraemer Boulevard, M/S W513, Brea, California 92821 for the procurement of SPRIworks Fragment Library Kit I-50. The sole source determination is based on the fact that National Institute of Health (NIH) is the nation’s medical research agency and the primary Federal agency conducting and supporting making medical discoveries that improve people’s health and save lives. The National Human Genome Research Institute (NHGRI), NIH Intramural Sequencing Center (NISC) is a centralized research facility for performing high-throughput, large-scale DNA sequencing and sequencing analysis. The Beckman SPRIworks Fragment Library System would enable NISC to reduce the extensive manual labor required in library preparation for the Illumina Genome Analyzers so the personnel can focus on other preparation work. This equipment has reduced cost in additional personnel, reduce errors from manual processing since the machine is fully automated and the reagents are pre-dispensed, and provide reproducible and consistent library output. Beckman Coulter’s SPRIworks Fragment Library Kit I contains all reagents and plastic consumables for use in the Beckman Coulter’s SPRIworks Fragment Library System, a fully automated instrument for the preparation of library samples for the Illumina Genome Analyzer. The reagents are pre-dispensed in a disposable cartridge. Each cartridge contains all of the reagents required to prepare a single library. In addition to the necessary enzymes and buffers for the preparation of a library, the cartridge reagents include an optimized size selection capability based on SPRI chemistry. SPRI is an acronym for Solid Phase Reversible Immobilization and are covered under US Patents 5,705,628, 5,898,071 and 6,534,262. SPRI chemistry is a novel chemistry in which nucleic acids are immobilized around the surface of the particles by controlling environmental reaction conditions such as pH, salts concentration and hydophobicity and hydrophilicity. The SPRIworks Fragment Library System I uses the patented Agencourt SPRI paramagnetic particle-based technology to generate fragment libraries from fragmented or sheared input DNA. The SPRIworks Fragment Library System automated method is performed on the SPRI-TE Nucleic Acid Extractor, using pre-measured reagents in a sealed reagent cartridge, and enables the construction of three library types: no size selection; 200-400 bp size selection; and 300-600 bp size selection. Contractor Requirements: • Library preparation must be completely processed in 4 hours. • Able to do at least 20 samples in one working day (8 hours). • Reagents provided pre-made, pre-dispensed and ready-to-go which reduces/eliminates mistakes and contamination. • All reagents and plasticware consumables must be contained in the kit. • Starting material should be no more than 3-5 µg. • Library preparation must be performed using Beckman Coulter’s SPRIworks Fragment Library System. Industry Classification (NAICS) Code is 334516, Analytical Laboratory Instrument Manufacturing, with business size standard 500 Employees. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001) and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-46 (October 29, 2010). This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this synopsis, by November 2, 2010, 7:30 a.m. Eastern Standard Time. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Inquires to this announcement, referencing synopsis number NHLBI-PB-(HG)-2011-05-DLM, may be submitted to the National Heart, Lung and Blood Institute, Office of Acquisition, Procurement Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-PB-(HG)-2011-005-DLM/listing.html)
- Place of Performance
- Address: NIH, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN02319586-W 20101031/101029234120-8a93f937134adaa0e4ab73d3560aa9a6 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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