SOLICITATION NOTICE
A -- IVIS Lumina II XR Instrument
- Notice Date
- 9/23/2010
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Veterans Affairs;VISN 9 ASC (90C);1639 Medical Center Parkway, Suite 400;Murfreesboro TN 37129
- ZIP Code
- 37129
- Solicitation Number
- VA24910RQ0425
- Response Due
- 9/29/2010
- Archive Date
- 10/29/2010
- Point of Contact
- Stacey M Chumney
- E-Mail Address
-
sing
- Small Business Set-Aside
- N/A
- Description
- Item Description Title: Imaging System Please provide a quote by email to Stacey.chumney@va.gov no later than 09/29/2010 3:00 pm. for the following: -Please specify if pricing is GSA or Open Market. -For Open Market items, indicate by line item otherwise all items will be considered as under contract. -Must provide Contract Number and Expiration Date. -Include any shipping costs that may apply, specify Origin or Destination -Must be Brand Name or Equal. -Preference: will be IAW FAR Part 13.003 (b)(1). Requirements for Imaging System Memphis VA Medical Center (614) Research and Development Maximal Sensitivity. We will need both luminescence and fluorescence capabilities. Due to the fact that our animal model of autoimmune arthritis can only be studied only in certain strains of mice and that all of these mouse strains have pigmented skin and fur, a factor which blocks light and reduces the detection of fluorescence and luminescence, we must have a level of sensitivity that can only be obtained from CCD cameras that are cooled to -90 degrees Centigrade. At lower temperatures, the CCD dark current and hence thermal noise is reduced, resulting in an improvement in sensitivity of about 33 fold. Also to increase the sensitivity further, we will need a lens aperture of at least f/0.95. We need a quantum efficiency of >80% maximum efficiency in the range of 500-700nm with a 1 CCD chip to collect more light from these dark-skinned mice and to provide a larger group of animals to be imaged at one time. Any change from these standards could reduce sensitivity to 0.2% of what we need. Also, the sensitivity of the camera due to this size chip allows lower X-ray exposure time and reduced radiation, important in the study of the immune system in our mouse model of autoimmune arthritis. We need a limit for the dose of radiation to less than 3mGy. Calibrated measurements for scanning. We must have calibrated data to eliminate day to day instrument variability from individual animal scans in order to measure effects on arthritis incidence and provide reproducible statistics. Units must be calibrated against NIST traceable radiance standards so that images measured in relative light units (CCD camera counts) can be converted into physical light units of surface radiance expressed as photons/second/cm2/steradian. Mid-high range filters. We must have precise and non-overlapping filter sets for near infrared imaging and relative spectral unmixing for collection and separation of data from dyes emitting at 647, 680 and 750, and autofluorescence. This will allow us to use several probes with different labels at the same time reducing animal numbers and costs. Other specifications. Instrument Physical Requirements: Imaging Chamber Dimensions: 46 cm x 46 cm x 51 cm (D x W x H) Gas anesthesia inlet and outlet ports for connection with gas anesthesia machine Heated sample stage: Ambient to 40 C High performance acquisition computer and minimum 20-inch, high-resolution flat screen monitor Operation manual Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum Light-tight imaging chamber 8 position emission filter wheels 10 position excitation filter wheels 5 filter wheel choices for a broad range of fluorescence applications LED lamps for photographic images Heated stage to maintain optimum body temperature Motor controlled stage, filter wheel, lens position, and f-stop The high sensitivity camera allows fast X-ray image acquisition times of 1-10 seconds reducing radiation exposure Radiation shielded Cabinet Exceeds standards set by the US FDA Center for Devices and Radiological Health (21 CFR-1020.40) Automated image integration to overlay with Bioluminescence, Fluorescence Gas Anesthesia Module Isoflurane vaporizer with independent flow control for proper gas delivery to induction chamber and the imaging chamber Isoflurane-absorbing disposable charcoal filters to absorb excess gas, as well as limit gas escaping into the surrounding laboratory environment Induction Chamber anesthetizes up to 5 adult mice or 2 rats simultaneously - Ventilates excess gas automatically when lid is opened - Raised animal floor maintains animal cleanliness during anesthetization 5-Port Anesthesia Manifold - Works within imaging chamber - Delivers gas to up to 5 adult mice or 2 rats - Ventilates waste gas from within imaging chamber - Matte black, non-glow construction protects data integrity - Autoclave safe - Transparent anesthesia manifold nose cones maintain proper animal positioning and ensure targeted gas delivery during imaging - Black rubber stoppers seal gas anesthesia manifold ports not used during anesthetization Workstation Workstation needs to fit in a 50 by 32 area and have easy-rolling casters for portability and lock-downs to prevent rolling. Support structure for instrument must be made of stainless steel. Software with a Graphical User Interface for In Vivo Imaging Software is required for a GUI based analysis of images consisting of superimposed photographic and luminescent images. The software needs to be able to create and manipulate spatial analysis tools to define regions of interest and to perform various measurements of the corresponding object, including counting photon emissions. The GUI needs to allow for storage of results in a dated electronic notebook, as well as image presentation and printing while maintaining image view. Absolute Determination of Light Source Intensity in Low Level Light Systems. Systems and methods to obtain and present imaging data in absolute units by using compensation factor to convert relative image data to absolute light intensity data. Software with easy-to-use graphical user interface for detection, quantification, analysis and storage of images taken. Needs to quantify absolute photon counts, and convert the data into false color images that will overlay on images of the animal so that the location of photon sources in the subject can be precisely identified. Must maintain an easily searchable digital lab book that records the camera settings and conditions for each image taken. Imaging chamber design that is extremely light-tight and made of low fluorescent background materials, so that the system may be used in laboratories at ambient light levels no need for a specially darkened room. Fluorescence system required so that both fluorescent and bioluminescent imaging techniques can be performed using a single system. Calibration device needed for determination and calibration of absolute photon intensity for standardized measurements from experiment-to-experiment. TOTAL COST: If you have any questions, please contact me at number below or by email. Thank you, /es/ Stacey Chumney Contracting Specialist VISN9 NCA Phone: 615-225-6956 Fax: 615-225-5432 Email: Stacey.chumney@va.gov
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