SOLICITATION NOTICE
66 -- High-Definition, fluorescence, In Vivo Optical Imaging System for the Observation and Quantification of Physiological Phenomena at the Cellular and Sub-Cellular Levels In Vivo in Laboratory Animals
- Notice Date
- 7/28/2010
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892
- ZIP Code
- 20892
- Solicitation Number
- N0I1690443
- Archive Date
- 8/27/2010
- Point of Contact
- Korriise L. LaRoche, Phone: 301-435-8782
- E-Mail Address
-
larochek@mail.nih.gov
(larochek@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a notice of intent, not a request for a proposal. A solicitation document will not be issued and proposals will not be requested. The National Institute on Drug Abuse (NIDA) intends to solicit, negotiate and award a purchase order on a noncompetitive basis to Visual Sonics, Inc., 3080 Yonge St. Suite 6100, Toronto, Ontario, Canada M4N, 3N1 for the Cellvizio LAB System Package. A major focus of NIDA IRP researchers is the identification of dynamic changes that occur in specific neuronal populations of the brain following acute and repeated administration of drugs of abuse and whether manipulations that prevent these attenuate compulsive drug-seeking and taking. Although multiphoton microscopy has been used in dynamic studies in vivo; it can only access the adult rodent brain down to about 500 um. Thus, one cannot image structures deeper than layer II of the cortex and imaging is accomplished in anesthetized animals. As such, the relevance of dynamic neuronal changes to a behavior (e.g. drug self-administration) cannot be determined. Until recently, in vivo access of deep structures has been limited to non-invasive imaging techniques, such as magnetic resonance imaging and positron emission tomography, which have significantly lower resolution and acquisition times compared with those of microscopy. Recently, a new technique called fibred fluorescence microscopy (FFM) has been developed which enables in vivo imaging of individual, fluorescently labeled neurons in regions that are inaccessible by other technologies. FFM is the only method currently available that enables visualization of neurons in deep bran regions, quantification of neuronal activity in the awake and behaving animals, and real-time quantification of changes in neuronal/axonal morphology that occur in the awake animal. It uses a probe consisting of optical micro-fibers arranged in a bundle and a laser scanning unit for excitation/emission of fluorescent probes. This is coupled to appropriate software for quantification of real time neuronal dynamics. Due to a lateral resolution well below 15 um of the probes, in situ morphology of neurons can be analyzed in real-time. In addition, bevelled and cone-shaped probes allowed repeated access to brain loci located to a depth of 6 mm below the surface of the skull, and provide real-time imaging of such regions at cellular resolution. Using this technique, it is now possible to image various neural structures in the living animal and quantify axonal degeneration/regeneration of nerves. Furthermore, calcium transients can be detected in individual neurons on a sub-second time scale in the awake animal enabling direct analysis of the relationship between cell activity and behavior over time and in response to a particular pharmacological manipulation. The Cellvizio® LAB system is a turn-key, high definition, fluorescence in vivo optical imaging system that enables researchers to observe and quantify physiological phenomena at the cellular and sub-cellular levels in vivo in laboratory animals. It is the only integrated platform on the market. It has the components necessary to achieve cellular resolution appropriate for in vivo imaging of the brain in behaving mice and rats. The acquisition is being conducted using FAR part 13.106-1(b)(1) procedures and is exempt from the requirements of FAR part 6, competition requirements. This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this synopsis. The determination by the Government not to compete the proposed acquisition based upon responses to this notice is solely with the discretion of the Government. Comments to this announcement, referencing synopsis number NOI1690443 may be submitted to the NIDA, Station Support Simplified Acquisitions Branch, 31 Center Drive, Bldg 31, Room 1B59, Bethesda, MD 20892-2080 and attention Korriise LaRoche, Contract Specialist, larochek@mail.nih.gov, on or before August 12, 2010 3:00pm.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/N0I1690443/listing.html)
- Record
- SN02220594-W 20100730/100728235249-3ae9c0114040af0e3bf5df6dff5828bb (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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