SOLICITATION NOTICE
B -- Analyses of mitochondria copy number, DNA methylation, and gastric cancer risk in the Shanghai Women’s Health Study
- Notice Date
- 8/25/2009
- Notice Type
- Presolicitation
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- NCI-90218-KM
- Archive Date
- 9/24/2009
- Point of Contact
- Karri L. Mares, Phone: 3014357774, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
maresk@mail.nih.gov, cr214i@nih.gov
(maresk@mail.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI), Division of Cancer Epidemiology and Genetics (DCEG), Occupational and Environmental Epidemiology Branch plans to procure on a sole source basis an analyses of mitochondria copy number, DNA methylation, and gastric cancer risk in the Shanghai Women’s Health Study from the Universita degli Studi di Milano, Molecular Epidemiology Laboratory (Epoca), Via San Barnaba 8, 20122 Milan, Italy. The services herein are being procured in accordance with the simplified acquisition procedures authorized by FAR Part 13. The North American Industry Classification System Code is 621511 and the business size standard is $13.5 million. The period of performance shall be twelve (12) months from date of award. Mitochondria are cellular organelles that perform multiple functions; chief among them is energy production through oxidative phosphorylation. During the process, reactive oxygen species are generated. Other functions of mitochondria include control of cell death, growth, integration of signals between mitochondria and nucleus, and various metabolic pathways. The mitochondria genome consists of a 16.6kb double-stranded DNA molecule, which is present at thousands of copies per cell in most tissues. Increased copy number of mitochondria DNA has been linked to exposure to environmental carcinogens such as cigarette smoke and benzene, whereas loss of the tumor suppressor p53 has been associated with mitochondria DNA depletion. Increased risk of certain cancers has been linked to decreased mitochondria DNA content in leukocyte DNA and tumor tissues. Little is known about the association of mitochondria DNA content and gastric cancer risk, although it has been shown that Helicobacter pylori, a gastric pathogen, can induce apoptosis through apoptosis-inducing factors from mitochondria. These observations suggest that mitochondria DNA content could play a role in gastric carcinogenesis, but data from epidemiologic studies with prospectively collected samples are largely unavailable. DNA methylation is an epigenetic mechanism that regulates chromosomal stability and gene expression, and is linked to both transcriptional silencing and activation. Abnormal DNA methylation patterns have been observed in many types of human tumors, since cells with abnormal DNA methylation acquire an overall gene expression pattern that favors proliferation and differentiation, leading to neoplastic transformation. In some cancers, such as gastric cancer, tumor-suppressor genes are more frequently inactivated by abnormal DNA methylation than by mutations. Recently, the presence of a methylation fingerprint of H. pylori infection in human gastric mucosa has been suggested. DCEG investigators and others have observed increased gastric cancer risk in relation to aberrant methylation in retrospectively collected leukocyte DNA, but data from prospective studies are sparse. The purpose of this contract is to perform analyses of mitochondria DNA content and global and gene-specific methylation in leukocyte DNA of stomach cancer cases and controls nested within a prospective cohort study. Specifically, the contractor shall perform mitochondria DNA content analysis on leukocyte DNA from 750 gastric cancer cases and control subjects from the Shanghai Women’s Health Study, perform bisulfite treatment of DNA, PCR amplification and pyrosequencing for methylation analysis on leukocyte DNA of 750 subjects, including global methylation (LINE-1 and Alu) and the promoter region of 3 genes to be determined by NCI, and deliver results for the mitochondria content analysis and methylation results including a technical report on all methods used in the analyses. The Molecular Epidemiology Laboratory, University of Milan, Italy is a leading molecular epidemiology laboratory that has acquired the expertise and resources critical for conducting analyses of epigenetic changes, including DNA methylation, telomere length, and mitochondria content, in large epidemiologic studies. This laboratory has conducted analyses of global DNA methylation in relation to gastric cancer risk in the Polish stomach cancer study and methylation in a few specific genes potentially relevant for gastric cancer in the Shanghai Women’s Health Study. They are uniquely qualified to carry on these analyses as a follow on to previous work related to the study. The conduction of the DNA methylation analyses in the same lab will allow NCI to simplify the control of laboratory operations and guarantee standardized quality control procedures. This will also enhance the comparability of results should NCI decides to conduct pooled analysis for more detailed evaluation of gene-environment interactions in the future. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI to determine if the party can meet this requirement. One (1) original and one (1) copy of the capability statement must be received in the contracting office by 11:00 a.m. ET on September 9, 2009. All questions must be in writing and can be faxed to 301-402-4513 or sent via email to Kerri Mares at maresk@mail.nih.gov. It is the vendor’s responsibility to ensure questions have been received. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractor must have valid registration and certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Application (ORCA). Please reference NCI-90218-KM on all correspondence.
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- Record
- SN01925306-W 20090827/090826000251-5e45f1fe642e054bdca449113f0dbd04 (fbodaily.com)
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