SOLICITATION NOTICE
66 -- Brand name Incu-Cyte FLR Microscope System including 24-Well Scratch Wound Module; 50 pack of 24-Well ImageLock plates; and Angiogenesis Module
- Notice Date
- 8/4/2009
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- NCI-90164-AV
- Archive Date
- 8/29/2009
- Point of Contact
- Ashley L. Virts,
- E-Mail Address
-
virtsa@mail.nih.gov
(virtsa@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI), Cancer Center Research (CCR), Pediatric Oncology Branch (POB) plans to procure the following brand name Incu-Cyte FLR Microscope System including 24-Well Scratch Wound Module; 50 pack of 24-Well ImageLock plates; and Angiogenesis Module manufactured by Essen Instruments, Inc.; 1156 Oak Valley Drive; Ann Arbor, Michigan 48108. The services herein are being procured in accordance with the simplified acquisition procedures authorized by FAR Part 13.106-(b) (1). The North American Industry Classification System Code is 334516 the business size standard is 500 employees. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the researcher to determine if the party can meet this requirement. One (1) original and one (1) copy of the capability statement must be received in the contracting office by 11:00 a.m. ET on August 14, 2009. All questions must be in writing and can be faxed to 301-402-4513 or sent via email to Ashley Virts: virtsa@mail.nih.gov. It is the vendor’s responsibility to ensure questions have been received. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractor must have valid, updated registration and certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Application (ORCA). Please reference NCI-90164-¬AV on all correspondence. Part I: Background information and description of requirements: The Pediatric Oncology Branch (POB) conducts an integrated research program designed to elucidate the cellular and tissue changes associated with specific stages of carcinogenesis, to detect genetic modifiers fundamental to cancer susceptibility and progression, to define the molecular mechanisms involved in the pathogenesis of major hum cancers, and to develop rational approaches for cancer prevention and treatment. Studies are performed in vivo in animal models, in vitro in cell and organ culture, and on tissues and cells obtained from human volunteers and cancer patients. Elucidating specific signal transduction pathways that involve in oncogenic transformation and finding specific inhibitors that attenuate cancer cell proliferation have been the focus of many researchers in the laboratory. Live cell imaging provides the researchers a powerful tool to study cell proliferation, migration, and cytotoxicity. The IncuCyte FLR Micrscope System and components are a compact, automated image platform that provides kinetic, non-invasive live-cell image for both phase and fluorescence. This system is a powerful tool for studying cell biology, including cell migration, proliferation, and cytotoxicity while closely monitoring cell morphology. The system will record images from different labwares with high throughput capacity, and accommodates multiple users for complicated experiment designs. The IncuCyte is useful for a range of both labeled and non-labeled applications. The system is designed to provide non-perturbing (label- free) kinetic growth curves using a built-in confluence algorithm and follow live-cell fluorescent reporters in time. The labor-free, automated image collection also provides a convenient method to digitally archive cell growth and morphology in real-time. Together, these tools have proven very useful for cell culture, QC, QA and as a cell-based assay optimization tool. The IncuCyte also provides a series of built-in processing metrics, kinetic, quantitative read-outs.for cell migration and cell proliferation, as well as co-culture angiogenesis. In addition, this system will allow for the observation of cell morphological changes during the treatment of inhibitors, follow the cell migration, and select specific inhibitors that can attenuate cancer cell proliferation while inducing terminal differentiation. The entire system is complete with the following: 1.IncuCyte Microscope system includes the following: Controller Unit, On-site Training and Installation 2.24-Well Scratch Wound Module includes the following: Wound Maker Tool, 2 boxes of Eppendorf Tips, 15 ImageLock plates, 2 Software Key Files, Microplate Tray. 3.1 each 50 pack of 24 Well ImageLock Plates 4.1 each Angiogenesis Module Delivery Date: 10 weeks from date of award. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION: Part II: Facts and Reasons to Justify Other than Full and Open Competition: Statutory Authority 1.Statute: This justification has been prepared under the authority of 41 U.S.C. 253 (c)(1) as set forth in 13.106-1 (b)(1) 2.Justification: Essen Instrument Inc is the only source known to the NCI with a system that not only provides live-cell image with high-throughput capacity, but also provides software to extract quantitative information from the collected data. The IncuCyteFLR combines high-definition phase contract microscopy and green fluorescent protein (GFP) technology as well as web-based software which allows multiple users to have access to their data from computers at their desks or even home. The IncuCyte microscope system and its web-based software are developed by Essen Instrument Inc. and there is no known similar product available from other companies. The IncuCyte FLR system is designed to provide continuous, time-lapse images of live cells within a cell culture incubator specifically designed by Essen. Space limitation within the lab is a major factor in this acquisition so multiple users can have access simultaneously with various vessel types, tray positions and initiation times. The IncuCyte FLR system is the only system known to the NCI that fits wtihin the existing incubator in the lab measuring 20”x 21”. For these reasons, no other entity is known within the NCI to provide supplies consistent with the needs of the lab. Salient Characteristics: •Analyze in real-time cell growth due to drugs, biogenetics and genetic manipulation •Associated software (included standard) enables investigators to generate movies •Fluorescence capability enables researchers to utilize GFP technology •Utilizes co-transfection of GFP and siRNA’s to monitor effects of the gene KO in the GFP positive population •Multi-well format/dynamic monitoring systems facilitates speed assessment of different genes •Non-invasive live-cell image for both phase and fluorescence •Accommodates multiple users for complicated experiment designs •Capable of a range of both labeled and non-labeled applications •System is designed to provide non-perturbing (label- free) kinetic growth curves using a built-in confluence algorithm and follow live-cell fluorescent reporters in time •Provides a convenient method to digitally archive cell growth and morphology in real-time •Holds various types and brands of cell culture vessels including dishes, flasks as well as 96 and 348 formatted microplates •Users can mix and match vessel types using three different tray positions while the cells remain stationary during image acquisition facilitating imaging on adherent as well non-adherent cells •Dimensions of system are 10" H by 18" W by 19" D which fit into the existing incubator in the lab (20” x
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