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FBO DAILY ISSUE OF SEPTEMBER 13, 2008 FBO #2483
SOLICITATION NOTICE

A -- Shotgun library construction of BAC clone

Notice Date
9/11/2008
 
Notice Type
Combined Synopsis/Solicitation
 
NAICS
621511 — Medical Laboratories
 
Contracting Office
Department of Agriculture, Agricultural Research Service, Pacific West Area Parlier, CA, San Joaquin Valley Sciences Center, 9611 S. Riverbend Ave., Parlier, California, 93648
 
ZIP Code
93648
 
Solicitation Number
AG9135S08A664
 
Response Due
9/16/2008
 
Archive Date
10/1/2008
 
Point of Contact
Saul R Garcia,, Phone: 559-596-2956
 
E-Mail Address
saul.garcia@ars.usda.gov
 
Small Business Set-Aside
N/A
 
Description
The USDA-ARS, Shafter Research and Extension center, Shafter, CA has a requirement for a shotgun library construction of BAC clones which will consist of 7.5x coverage sequencing of 20 BAC clones which will be used in a genetics research and breeding program. The entire unit will consist of shotgun library, plate and array for 20 BAC's and the sequences for 20 BAC's. Entire project will consist of the construction of a small-insert library of 20 BAC clones (estimated insert size of 137kb), sequence to a desired level of coverage. Small-insert DNA fragments will be generated by extracting BAC DNA from BAC clones and subjecting to hydroshearing (random fragmentation). Fragments between 3 and 5 kb will be size selected by gel electrophoresis, subject to end-repair, cloned into the hi-copy plasmid based cloning vector pBlueskriptIIKSII+ and then electorprorated into E. coli DH108 cells. Transformants will be selected on LB plates containing carbenicillan, X-Gal and IPTG. White recombinant colonies will be picked robotically using the Genetix Q-bot and stored as individual clones in Genetix 96-well microtiter plates as glycerol stocks at -80°C. Clones will be miniprepped and DNA sequencing will be performed with BigDye version 3.1 in both the forward and reverse directions using the universal priming sites on the vector. DNA will be accumulated on an ABI 3730 xl DNA analyzer. A RFQ is not available for this requirement. The USDA ARS, intends on negotiation with Clemson University Genomics Institute (CUGI) on a sole source basis. See numbered note 22
 
Web Link
FedBizOpps Complete View
(https://www.fbo.gov/?s=opportunity&mode=form&id=b3dd24499d8897e0063ffcde5b6e0633&tab=core&_cview=1)
 
Place of Performance
Address: 17053 N. Shafter Ave, Shafter, California, 93263, United States
Zip Code: 93263
 
Record
SN01667043-W 20080913/080911224711-b3dd24499d8897e0063ffcde5b6e0633 (fbodaily.com)
 
Source
FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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