SPECIAL NOTICE
66 -- Hyperspectral Confocal Fluorescence Microscope and Multivariate Analysis Algorithms and Software Available for Technology Transfer
- Notice Date
- 5/20/2008
- Notice Type
- Special Notice
- NAICS
- 334111
— Electronic Computer Manufacturing
- Contracting Office
- Department of Energy, Sandia Corp. (DOE Contractor), Sandia National Laboratories, PO Box 5800, MS: 0115, Albuquerque, New Mexico, 87185
- ZIP Code
- 87185
- Solicitation Number
- 08_312
- Response Due
- 6/20/2008
- Point of Contact
- Rudolph R Griego, Phone: 505-844-7522
- E-Mail Address
-
rrgrieg@sandia.gov
- Small Business Set-Aside
- N/A
- Description
- Sandia National Laboratories has designed and constructed a new hyperspectral confocal fluorescence microscope. This patent-pending technology has been combined with Sandia’s unique and proprietary multivariate algorithms and software to form a complete system for the extraction of quantitative image information from the hyperspectral images. The hyperspectral microscope uses laser excitation and collects 512 spectral emission wavelengths at each voxel in the image over the spectral range from 500 to 800 nm at a spectral resolution of 1-3 nm and at imaging rate of 8300 spectra/sec (with extension to 64,000 spectra/sec in the near future). Our proprietary multivariate curve resolution (MCR) software allows us to very rapidly “discover” all emitting fluorescence species in the image and to determine their relative concentrations throughout the image without any a priori information. With this new system, many fluorophores can be monitored simultaneously without cross talk to achieve higher throughput, greater quantitative accuracy, and increased reliability. The hyperspectral microscope has been used for multiplexed live-cell imaging at diffraction-limited spatial resolutions in a variety of biological applications. Included in the hyperspectral imaging system is spectral image viewing software to view both the raw image data as well as the results from the MCR analyses. Sandia is interested in partnering with microscope manufacturers through licensing agreements for the commercialization of this technology. Sandia anticipates hosting a workshop in late July to showcase this technology and to allow interested parties the opportunity to view this technology in operation. Please respond with interest to RR Griego, fax (505) 844-8011; rrgrieg@sandia.gov Selected References: 1.Michael B Sinclair, David M. Haaland, Jerilyn A. Timlin, and Howland D.T. Jones, “Hyperspectral Confocal Microscope,” Applied Optics 45, 6283-6291 (2006). 2.Willem F.J. Vermaas, Jerilyn A. Timlin, Howland D.T. Jones, Michael B. Sinclair, Linda T. Nieman, Sawsan Hamad, David K. Melgaard, and David M. Haaland, “In vivo Hyperspectral Confocal Fluorescence Imaging to Determine Pigment Localization and Distribution in Cyanobacterial Cells,” Proceedings of the National Academies of Sciences, 105, 4050-4055 (2008). 3.P. G. Kotula, M. R. Keenan, and J. R. Michael, “Automated Analysis of SEM X-Ray Spectral Images: A Powerful New Microanalysis Tool,” Microsc. Microanal. 9, 1–17 (2003). 4.Mark H. Van Benthem, Michael R. Keenan, and David M. Haaland, “Application of Equality Constraints on Variables during Alternating Least Squares Procedures,” Journal of Chemometrics, 16, 613-622 (2002). 5.Mark H. Van Benthem and Michael R. Keenan, “Fast algorithm for the solution of large-scale non-negativity-constrained least squares problems,” J. Chemometrics 18, 441–450 (2004).
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