SOLICITATION NOTICE
66 -- Multilazer upgrade
- Notice Date
- 8/24/2007
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- Reference-Number-NCI-70150-NG
- Response Due
- 9/10/2007
- Archive Date
- 9/25/2007
- Description
- The National Cancer Institute (NCI) plans to procure on a sole source basis; a multilaser upgrade to the government owned Nikon LCM 2000 laser confocal microscope. The upgrade will include a laser operating at 405 nm wavelength to work in concert with the three existing lasers and capable of activating the DNA stain 4',6-diamidino-2-phenylindole (DAPI) in a manner suitable for fluorescent microscopy, with Microcosm Inc., 6430 Dobbin Road, Suite B; Columbia, Maryland 21045. The supplies and services herein are being procured in accordance with the simplified acquisition procedures authorized by FAR Part 13.106. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. The following salient characteristics apply: Laser: - Operates at 405 nm or shorter wavelength - Activates the dye stain 4',6-diamidino-2-phenylindole (DAPI); DAPI 405 nm laser shall enable the use of Hoechst, CellTracker Blue, Alexa 405 conjugated proteins, Pacific Blue conjugated proteins, and coumarin dyes as cell stains. - Incorporation into Nikon PCM 2000 for simultaneous operation with existing lasers - Enables Nikon PCM 2000 to acquire three channel images Nikon PCM 2000: - optical parts of the Nikon PCM 2000 to provide transmission path of 405 nm laser energy to the sample - laser assembly into cabinet approximately 24 x 18 x 8 inches - photomultiplier tubes (PMTs) to record intensity of 405 nm light Justification: NCI currently has a Nikon LCM2000 confocal microscope which has been the workhorse on projects related to analyzing -H2AX foci. However, in its present state, the microscope is not capable of obtaining images with DAPI, the best fluorescent dye for visualizing DNA. With an excitation band of 395-410 nm, DAPI is commonly excited with UV lasers. This laser's emission is at the upper end of the DAPI excitation band, but this has been shown to adequately activate the molecule. In addition to DAPI, the 405 nm laser will further extend our capabilities to visualize Hoechst, CellTracker Blue, Alexa 405 conjugated proteins, Pacific Blue conjugated proteins, and coumarin dyes. The aforementioned will provide the capabilities stated above. Benefit The upgrade will allow the NCI to make observations that cannot be made on the present equipment. Two types of observations are particularly important. The first is the ability to visualize nuclear DNA with DAPI, which helps differentiate different chromatin environments of the DNA, such as eu- and hetero-chromatin. The ability to make such observations will permit the NIC to study the differential repair of DNA DSBs in the various chromatin subregions, and help better understand the impact of DSB nuclear location on its potential for DAN damage and cancer industion. Secondly, the addition of a laser for DAPI will permit the NCI to visualize at least two and perhaps three other dyes, enabling us to design experiments that measure the rate of accumulation of DNA repair proteins to these foci. Addition of the 405 nm laser will provide higher quality images thus allowing us to perform better experiments. Microcosm Inc is the only source known to the NCI that is the sole Nikon factory-authorized service provider for the Nikon PCM 2000. The ML4 upgrade is custom-made by Microcosm and modified to the customers needs and specifications. This is not a solicitation for competitive quotations. However, if any interested party believes they can meet the above requirement, they may submit a statement of capabilities. All information furnished shall be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the above unique specifications described herein. An original and one copy of the capability statement must be received in the NCI contracting office by 1:00 PM ET on September 10, 2007. All questions must be in writing and can be faxed (301) 402-4513 or emailed to Malinda Holdcraft, Contract Specialist at holdcram@exchange.nih.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the Central Contractor Registration (CCR) www.ccr.gov and the Online Representations and Certifications Applications (ORCA), http://orca.bpn.gov. No collect calls will be accepted. Please reference NCI-70150-NG on all correspondence.
- Record
- SN01384035-W 20070826/070824220652 (fbodaily.com)
- Source
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