Loren Data's SAM Daily™

FBO DAILY ISSUE OF APRIL 08, 2007 FBO #1959
SOURCES SOUGHT

A -- Evaluation of the acute and persistant/resting memory phase responses of rabbits to rPA vaccine

Notice Date

4/6/2007
 

Notice Type

Sources Sought
 

Contracting Office

US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014
 

ZIP Code

21702-5014
 

Solicitation Number

W81XWHVACCINE
 

Response Due

4/25/2007
 

Archive Date

6/24/2007
 

Small Business Set-Aside

N/A
 

Description

" THIS IS NOT A SOLICITATION ANNOUNCEMENT. THIS IS A SOURCES SOUGHT SYNOPSIS ONLY. THE PURPOSE OF THIS SOURCES SOUGHT SYNOPSIS IS TO GAIN KNOWLEDGE OF POTENTIAL QUALIFIED SOURCES FOR PLANNING PURPOSES ONLY FOR THE U.S. ARMY MEDICAL RESEARCH ACQUISI TION ACTIVITY (USAMRAA), FORT DETRICK, MARYLAND. USAMRAA is seeking a Contract Research Organization (CRO) to perform the attached Statement of Work. This announcement is not a formal solicitation and is not a request for proposal. No contract will be a warded from this announcement. No reimbursement will be made of any costs to provide information in response to this announcement or any follow up information requests. Availability of any formal solicitation will be announced under a separate announceme nt. This synopsis is for information and planning purposes only and is not to be construed as a commitment by the Government. Each response must reference the sources sought title. All proprietary information should be marked as such. Responses will be held confidential, if requested. Please submit fact sheets to the attention of: USAMRAA, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014. Fact sheets may be e-mailed to: Anthony.Petrore@us.army.mil. Responses must be received no later than 25 April 2007, by 4:00 P.M. Eastern Standard Time. Original Point of Contact Anthony Petrore, Contract Specialist, 301-619-1342 Email your questions to US Army Medical Research Acquisition Activity at anthony.petrore@us.army.mil. Statement of Work Evaluation of the acute and persistant/resting memory phase responses of rabbits to rPA vaccine 1. Purchase 16 New Zealand white rabbits, 8 males and 8 females, at 2.7-3.1 kg each. Allow animals to acclimatize for 1 week, if necessary. 2. Prebleed 16 rabbits prior to the start of the experiment for (i) 1 ml of serum and (ii) 14 ml of blood to isolate PBMCs. These cells will serve as baseline values and negative controls for the lymphocyte proliferation assay, CD4/CD8/CD3 FACS, and real -time RT-PCR. 3. One week after the pre-bleed, inject 50 ?g rPA/Alhydrogel vaccine intramuscularly (0.5 ml each) into 12 rabbits (6 male and 6 female). Inject 4 rabbits (2 male and 2 female) with PBS/Alhydrogel (age-matched controls). Vaccine will be supplied by USAM RIID. 4. Divide the rabbits into two groups A and B each of 6 rPA-immunized rabbits (3 males and 3 females) and 2 controls (1 male and 1 female). 5a. Group A (acute memory phase); A. Bleed 8 rabbits on days 2, 4, and 6 for 2 ml of blood. Send frozen serum to USAMRIID. B. Sacrifice 8 rabbits on day 7 by exanguination and harvest and purify PBMCs. a. Lymphocyte proliferation assay using cultured PBMCs stimulated with PA. Include positive and negative controls. USAMRIID will provide PA. b. Real time RT-PCR for IFN-? and IL-4 RNA using the PBMCs. Include reference standard (i.e. glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or ?-actin). Published GenBank rabbit sequences for IFN? accession number AB010386, for IL-4 accession number AF 169169, for GAPDH, accession number NM001082253, and for ?-actin, accession number AF309819. (Please describe the detection chemistries, instrumentation, and quantification method to be used). c. FACS on cultured and rPA-stimulated PBMCs to identify/separate CD4/CD8/CD3 T cells. Include positive and negative controls. USAMRIID will provide PA. 5b. Group B; Bleed 8 rabbits on days 2, 4, 6, 8, 10, 12, 14, 16, 18, and 21 for 2 ml of blood. Send frozen serum to USAMRIID. The blood volume of 2.7-3.1 kg rabbits is about 56 ml/kg or about 150 ml at a minimum. It is safe to remove 7.5% blood on a w eekly basis (11 ml). Thus, each week the volume would be 6-8 ml, within the accepted guidelines. 6. Group B; On day 21, boost im 6 rabbits with rPA/Alhydrogel and 2 rabbits with PBS/Alhydrogel as used in the primary injection. 7. Group B; Bleed 8 rabbits on days 2, 4, 6, 8, 10, 12, 14, 16, and 18 after the booster injection for 2 ml of blood. Send frozen serum to USAMRIID. 8. On day 98 (77 days after the booster injections) for each of 8 rabbits, A. Collect 3 ml of blood in a serum tube. Send frozen serum to USAMRIID. B.. Sacrifice rabbits by exanguination and harvest and purify PBMCs. a. Lymphocyte proliferation assay using cultured PBMCs stimulated with PA. Include positive and negative controls. USAMRIID will provide PA. b. Real time RT-PCR for IFN-? and IL-4 RNA using the PBMCs. Include reference standard (i.e. glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or ?-actin). Published GenBank rabbit sequences for IFN? accession number AB010386, for IL-4 accession number AF 169169, for GAPDH, accession number NM001082253, and for ?-actin, accession number AF309819. c. FACS on cultured and rPA-stimulated PBMCs to identify/separate CD4/CD8/CD3 T cells. Include positive and negative controls. USAMRIID will provide PA. 9. Per diem for 8 rabbits 21 days and 8 rabbits for 112 days. 10. Serum can be sent in two shipments; after the final bleed of the boosted animals (day 39) and after the final sacrifice bleed (day 98). Group Vaccination Bleed for serum Bleed for serum post booster Sacrifice for PBMCs A Day 0 Days 2, 4, & 6 n/a Day 7 B Days 0 & 21 Days 2, 4 ,6, 8, 10, 12, 14, 16, 18, & 21 Days 2, 4 ,6, 8, 10, 12, 14, 16, &18 Day 98 Purchase 16 rabbits Pre-bleed 16 rabbits Bleed for control PMBCs 16 rabbits IM injections- (1 x 8) + (2 x 8) = 24 Sample Bleeds- (3 x 8) + (19 x 8) = 176 Sacrifice bleed and isolate PBMCs- 16 rabbits Lymphocyte proliferation assay- 3 x 16 = 48 Real time RT-PCR- 3 x 16 = 48 FACS- 3 x 16 = 48 Per diem 8 x 21 days + 8 x 112 days =
 

Place of Performance

Address: US Army Medical Research Acquisition Activity ATTN: MCMR-AAA, 820 Chandler Street Frederick MD

Zip Code: 21702-5014

Country: US
 

Record

SN01268301-W 20070408/070406225716 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
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