SOLICITATION NOTICE
66 -- Electric Cell-Substrate Impedance Sensing System
- Notice Date
- 9/1/2006
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
- ZIP Code
- 20852
- Solicitation Number
- Reference-Number-RFQ-NCI-60186-NV
- Response Due
- 9/8/2006
- Archive Date
- 9/23/2006
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Angiogenesis Core Facility (ACF) plans to enter into a sole source procurement for a Electric Cell-Substrate Impedance Sensing System ECIS 9600 Applied Biophysics Inc., 1223 Peoples Avenue, Troy, New York 12180. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. This acquisition will be processed under FAR Part 12 ? Acquisition for Commercial Items. Applied Biophysics Inc. is the only source known to the NCI researcher that has a electric cell-substrate impedance sensing system that has a high field module that allows one to carry out automatic wound healing assays without opening the incubator door which is required for their research and detailed below in their JOFOC. However, if any interested party believes it can perform requirement as detailed in the JOFOC they may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can perform this requirement. An original and one copy of the capability statement must be received in the contracting office by 1:00 pm EDT, on September 8, 2006. Faxed and emailed capability statements are NOT authorized. All questions must be in writing and may be emailed to Debbie Moore at dm170b@nih.gov or faxed (301) 402-4513. It is the vendor?s responsibility to call (301) 402-4509 and verify that questions were received. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award from the NCI contractors must be registered in the Online Representations and Certifications Applications (ORCA). Please refer to http://orca.bpn.gov in order to register. In addition, contractors must be registered in the Central Contractor Registration (CCR) www.ccr.gov. No collect calls will be accepted. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION Part I: Background information and Description of Acquisition Title: ECISTM Model 9600 (Electric Cell-Substrate Impedance Sensing System) an automated method to monitor cell behavior Purpose/Brief History: The ACF has a need to acquire cutting edge technology for the study of the behavior of cells involved in the biology of tumors, such as endothelial (microvascular and lymphatic) cells, pericytes, smooth muscle cells, tumor cells, inflammatory cells, etc. The ECIS Model 9600 is a high throughput fully automated instrument used to reproducibly quantify growth, migration, morphological changes, cell invasion, cell-ECM interactions among others in living cells. This noninvasive system is based on the real time measurement of impedance of small active gold electrodes built in each well where cells are grown. The systems can be also programmed to apply elevated fields for cell migration measurements via an automated wound-healing assay. In addition, high fields can be used at shorter durations to electroporate membrane-impermeable compounds (such plasmids, siRNA, etc) into cells. Minimal training is required when operating the instrument for research purposes in a laboratory environment. Existing technologies in the laboratory such as cell culture, migration assay, wound healing assay etc, are compatible with this technology which enhances our ability to quantify otherwise hard to measure biological events. PART II: Facts and reasons to Justify Other than Full and Open Competition Statutory authority FAR 13.106(b)(1) b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). Justification: Applied Biophysics Inc. is the only company which makes a electric cell-substrate impedance sensing system that meets the following specifications which are required fore the ACF?s research: 1) The ECIS 9600 6x16 is capable of automatically monitoring up to 96 tissue culture wells and of returning quantitative information regarding the impedance of small 250-micrometer electrodes used as substrates for cell growth. The system can be run at 3 different preset AC frequencies and monitors the voltage across the active electrodes with a full set of data sampling completed in 21 seconds. Custom software included with the system runs on a Windows platform and both controls data acquisition and analysis including plotting of the data time series. 2) The software includes the functionality of reading a single 96 well plate (9600) as well as a second set of software for multiple lab use (6x16 software) for monitoring six sets of 16 wells independently at the same time. The instrument comes complete with the basic instrumentation, a dedicated personal computer, 7 array holders for the incubator space, and a supply of ECIS consumable arrays (unless otherwise renegotiated). 3) The ECIS 9600 6x16 instrument can be used to monitor any animal cell that anchors to its substratum. Most cell types can be employed in ECIS-based assays for cell proliferation, cell attachment and spreading to ECM proteins, extravasion, signal transduction, and cell migration. 4) Other instruments that measure TER (transepithelial resistance) are designed for extremely tight epithelial layers and only make single point measurements; whereas, ECIS is not restricted to tight layers and can monitor any adherent cell type in monolayer or sparse culture with continuous, real-time measurements. 5) The ECIS 9600 6x16 system also has an optional high field module that allows one to carry out automatic wound healing assays. There is no other source of this technology on the market today that can accomplish a fully automated wound healing / migration assay in real time without opening the incubator door.
- Record
- SN01132497-W 20060903/060901220545 (fbodaily.com)
- Source
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