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FBO DAILY ISSUE OF JUNE 23, 2004 FBO #0940
SOLICITATION NOTICE

66 -- AKTA FPLC System

Notice Date

6/21/2004
 

Notice Type

Solicitation Notice
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Research Contracts Br., 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
 

ZIP Code

20852
 

Solicitation Number

NCI-40084-NG
 

Response Due

7/6/2004
 

Archive Date

7/21/2004
 

Point of Contact

Malinda Holdcraft, Purchasing Agent, Phone (301) 402-4509, Fax (301) 402-4513, - Renita Smith, Contract Specialist, Phone 301-496-8612, Fax 301-480-0241,
 

E-Mail Address

holdcram@exchange.nih.gov, rs442i@nih.gov
 

Description

The National Institutes of Neurological Disorders and Stroke (NINDS), Neurodegeneration and Aging Unit (NAU), Neurogenetics Branch (NB) plans to procure an AKTA FPLC FRAC950 system from Amersham Biosciences, 800 Centennial Avenue, Piscataway, New Jersey 08854. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. The supplies and services herein are being procured in accordance with the simplified acquisition procedures authorized by FAR Part 13. The Neurodegeneration and Aging Unit studies the underlying molecular mechanisms of various neurodegenerative disorders. One project in the lab focuses on understanding the function of a gene involved in Down's syndrome. NINDS, NAU is attempting to purify the protein for X-ray crystallography, and thus requires the need to purchase an FLPC system to purify our protein of interest. The laboratory requires the ability to purify biomolecules from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems and other sources. The system must be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. Requirements: 1) AKTA FPLC system shall require a flow rate range of .05-20mls/min at pressures ranging from 0-700 psi; 2) System must be a two-pump gradient system, with dynamic mixing, as evidence suggests that this configuration produces the highest accuracy and precision in gradient formation; 3) System pumps shall allow operation of the system at zero backpressure to ensure system compatibility with standard low pressure chromatography supports often used in affinity chromatography and other techniques; 4) Controller must allow programs to be time-based, volume-based, or column-volume based, and must allow user intervention of a running method (e.g., change of flow rate) without stopping or pausing the run; 5) Entire wetted flow path of the system must be inert to halides; 6) System must include a fraction collector which is fully integrated into the system and fully controllable via the system control software. Installation and training must be provided by the vendor, at no additional cost. The Amersham Bioscience AKTA FPLC system is the only LC system known to NINDS which meets the control and resolution requirements describe above. Amersham Bioscience's AKTA FPLC system offers accurate and precise control of salt gradient, motorized valves for column selection, buffer selection, and multi-dimensional chromatography, control up to 9 motorized valves are possible with this system, thus maximizing time efficiency. In addition, the system is specified for use at 4 C, allowing purification of proteins at a suitable temperature. This is not a request for competitive quotation. However, if any interested party believes it can perform the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform this requirement. Capability statements must be received in the contracting office by 1:00 PM EST, July 6, 2004. If you have any questions they must be submitted in writing to Malinda Holdcraft, Purchasing Agent, at holdcram@exchange.nih.gov or by fax 301-402-4513. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement.
 

Place of Performance

Address: Bethesda, MD

Zip Code: 20892

Country: USA
 

Record

SN00606931-W 20040623/040621211737 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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