SOLICITATION NOTICE
R -- Professional, Administrative, and Management Support Services
- Notice Date
- 9/4/2002
- Notice Type
- Solicitation Notice
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, MD, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-PS-2003-002
- Point of Contact
- Deborah Coulter, Purchasing Agent, Phone (301) 435-0366, Fax (301) 480-3345, - Debra Hawkins, Chief, Procurement Section, Phone (301) 435-0366, Fax (301) 480-3345,
- E-Mail Address
-
dc143b@nih.gov, dh41g@nih.gov
- Description
- The National Heart, Lung, and Blood Institute (NHLBI) intend to negotiate on a sole source basis with Dr. Jeung-Shan Han, 12639 Georgia Avenue #101, Silver Spring, Maryland, 20906 to conduct research in the NHLBI, Laboratory of Molecular Immunology, to examine the effects on histone acetylation as well as histone acetylase/deacetylase activities and with mast cell lines and examine the effects of glucorticoids on functional response of the cells to antigen and other stimulants. Dr. Han has conducted extensive research studies of histone acetylation. The research examined the effects of glucocorticoids on histone acetylation in response to antigen stimulation in mast cell lines by use of assay systems established in Dr. Han?s laboratory. Similar studies were conducted in cells transfected with mutated forms of the glucocorticoid receptor using classical and novel corticosteroid ligands, and it was determined whether or not the histone deacetylase inhibitors that were discovered by Dr. Han (e.g., apicidin) suppress the inhibitory actions of glucocorticoids in mast cells. The work of Dr. Han?s laboratory has shown that glucorticoids such as dexamethasone suppress all functional responses in antigen-stimulated mast cells although certain responses (such as production of cytokines) are especially sensitive to the inhibitory actions of glucocorticoids. In order to obtain the knowledge of the methodologies used in Dr. Han?s research an award to any other source would not be advantageous to the Government, and would result in a substantial increase of cost to the Government that is not expected to be recovered through competition. The Statement of work is as follows: The Laboratory of Molecular Immunology (LMI) is conducting research studies of histone acetylation and recent results has shown that the glucocorticoids suppress activities of several transcription factors by interfering with histone acetylation thereby preventing access of the transcription factors to the appropriate cytokine gene promoter. The LMI would like continue and expand their research in this area by utilizing the methodologies developed by Dr. Han in his research of histone acetylation. Independently and as an agent of the Government, the Contractor will conduct experiments with mast cell lines and examine the effects of glucorticoids on functional response of the cells to antigen and other stimulants. In addition he will examine effects on histone acetylation as well as histone acetylase/deacetylase activities by procedures that he has established in his own laboratory and as necessary instruct other laboratory members on these procedures. Specifically the Contractor shall: 1) Assess the extent of histone acetylation (and the associated histone acetylase/deacetylase activities) by procedures established in his laboratory in antigen-stimulated cells in RBL-2H3 cell line. Compare the extent of acetylation with that induced by mitogenic stimulants (Stem cell factor and IL-3) in mast cell lines dependent on these factors. 2) If as expected acetylation is stimulated determine the effect of dexamethasone on this stimulation. Functional responses such as secretion of granules and production of cytokines will be measured to assure that the effects of antigen and dexamethasone are optimized. Assistance will be provided as necessary. 3) Similar studies (as in item 2) will be conducted in cells transfected with mutated glucocorticoid receptors to determine whether the effects of glucocorticoids are due to transrepression or transactivation. The constructs for these mutants have been prepared and tested for expression. 4) Studies as outlined in items 2 and 3 will be conducted with ?dissociated steroids? and histone deacetylase inhibitors such as apicidin. 5) Establish procedures required for item 1 by teaching other laboratory workers how to run these procedures. In addition advise other workers on ongoing projects (particularly on projects related to phospholipase D) that might benefit from his experience. The government will provide on-site laboratory space, furnish all necessary reagents, laboratory equipment, and computer facilities as needed. Assistance will be provided by laboratory members who are conducting studies of the glucocorticoids in mast cell lines. The period of performance is approximately eleven months from the date of award. The contractor will work independently coordinating his efforts with the Project Officer. The Government intends to award by October 1, 2002. The acquisition is being conducted under simplified acquisition procedures and is exempt from the requirements of FAR Part 6. The North American Industry Classification System (NAICS) is 541990 and Standard Size is $6.0 million. This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this requirement. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Inquiries to this announcement, referencing solicitation number NHLBI-PS-2003-002 may be submitted to the National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, Building RKL2, Suite 6042, 6701 Rockledge Drive, Bethesda, Maryland 20892-7902, Attention Deborah Coulter, Purchasing Agent. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency.
- Record
- SN00157341-W 20020906/020904213416 (fbodaily.com)
- Source
-
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