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65 -- 65-iCycler iQ Real Time Detection System

Notice Date
Notice Type
Solicitation Notice
Contracting Office
Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Research Contracts Br., 6120 Executive Blvd. EPS Room 604, Rockville, MD, 20852
ZIP Code
Solicitation Number
Response Due
Archive Date
Point of Contact
Cynthia Brown, Purchasing Agent, Phone (301) 496-8608, Fax (301) 402-4513, - Caren Rasmussen, Lead Contract Specialist, Phone (301) 402-4509, Fax (301) 402-4513,
E-Mail Address
cb106x@nih.gov, rasmusc@mail.nih.gov
The National Cancer Institute(NCI), Laboratory of Biochemistry plans to procure an iCycler iQ Real Time Detection System in accordance with the Simplified Acquisition Procedures authorized in FAR Part 13. This is a combined synopsis/solicitation for commercial items, prepared in accordance with the format in the FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation, No RFQ-NCI-20049-NQ, includes all applicable provisions and clauses in effect through FAR FAC 01-07. The North American Industry Classification Code is 334516, and the business size standard is 500 employees. Researchers of the Laboratory of Biochemistry, frequently use the polymerase chain reaction (PCR) to quantify nucleic acid (RNA and DNA) concentrations in samples during research into the fundamental mechanism of cellular processes related to cancer causes and prevention. Currently, the Laboratory of Biochemistry is using end-point reactions combined with gel electrophoresis of final products to measure RNA and DNA concentrations. Due to the large number of samples, and technical issues having to do with the PCR reaction itself, end-point analysis is compromised with regards to reliability and reproducibility. The use of an instrument to monitor PCR amplification in real-time, using fluorescent probes, eliminates these problems. The NCI requires an iCycler iQ Real Time Detection System with the following minimum capability specifications and the mandatory specifications: 1) the ability to analyze up to 96 samples at one time; 2) the ability to detect up to four different fluors at one time, with minimal spectral overlap; 3) an instrument with a halogen (wide spectrum) light source with excitation and emission filters; 4) the ability to use Taqman, molecular beacon and SyBr Green probes in a simple and user-transparent manner, with software to aid in the design of all probes; 5) signal application for optimum sensitivity in detecting samples with low target concentrations; 6) mandatory requirements: sample block with thermal gradient capability, to minimize reaction optimization time (particularly important, as the instrument will be used by multiple users with multiple probe combinations, each of which must be optimized with regards to reaction temperature). Offers shall demonstrate that their offered product meets the mandatory and minimum capability specifications herein in order for all other specifications to be considered. Offeror must provide descriptive literature, warranties, and/or other materials in sufficient detail to demonstrate that their offer meets the foregoing requirements. The literature furnished must also show the item to which the specifications herein pertain to. The anticipated purchase order will be awarded to the responsible offeror with the lowest price offer that fully meets the requirements of this solicitation. The offer should include a fixed price for all equipment, delivery, and other applicable charges. In addition to the aforementioned descriptive literature, offers must also be accompanied by a completed and signed NCI Representations and Certifications, and other Statements of Offerors or Quoters (Simplified Acquisitions). The award document will incorporate the requirement of FAR 52.213-4, Terms & Conditions-Simplified Acquisitions. Full text copies of these documents maybe obtained from Cynthia Brown, Purchasing Agent, at (301-402-4509 ). Offers must be submitted on an SF-18 that is signed by an authorized representative of the offeror and include a compete "Schedule of Offeror Supplies/Services". The offer should include a fixed price for all equipment delivery installation, training, and other applicable charges. Offers and related materials must be received in this office by 2:00 PM EST (local Washington, DC time) on May 30, 2002. No collect calls will be accepted. Please cite the solicitation number on any and all correspondance
Web Link
Link to FedBizOpps document.
SN00080515-F 20020523/020521213159 (fbodaily.com)
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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